Role of SH3GLB1 in the regulation of CD133 expression in GBM cells.

ABSTRACT:

Background

Glioblastoma (GBM), a malignant brain tumor, has poor survival outcomes due to recurrence or drug resistance. We found that SH3GLB1 is a crucial factor for cells to evade temozolomide (TMZ) cytotoxicity through autophagy-mediated oxidative phosphorylation, which is associated with CD133 levels. Therefore, we propose that SH3GLB1 participate in the impact on tumor-initiating cells (TICs).

Methods

The parental, the derived resistant cell lines and their CD133⁺ cells were used, and the levels of the proteins were compared by western blotting. Then RNA interference was applied to observe the effects of the target protein on TIC-related features. Finally, in vitro transcription assays were used to validate the association between SH3GLB1 and CD133.

Result

SUBMITTER: Chien CH 

PROVIDER: S-EPMC10391956 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature