Cigarette smoking exposure disrupts the regenerative potential of dental pulp stem cells.

ABSTRACT:

Introduction

Smoking is known to alter the regenerative and immunomodulatory properties of many types of mesenchymal stem cells (MSCs). This study investigates the impact of cigarette smoke exposure on the regenerative potential of dental pulp stem cells (DPSCs).

Methods

DPSCs were treated with various doses of cigarette smoke condensate (CSC) or nicotine. Cell proliferation and survival were evaluated by a water-soluble tetrazolium salt (WST-1) and a survival assay. DPSC migration, cytokine expression, mutagenesis, and the signaling pathway were also measured during CSC and nicotine treatment.

Results

Low concentrations of CSC and nicotine did not impair cell proliferation, but higher concentrations reduced cell proliferation. CSC and nicotine could impede DPSC survi

SUBMITTER: Hardin LT 

PROVIDER: S-EPMC10392041 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature