The Prc and CtpA proteases modulate cell-surface signaling activity and virulence in Pseudomonas aeruginosa.

ABSTRACT: Cell-surface signaling (CSS) is a signal transfer system of Gram-negative bacteria that produces the activation of an extracytoplasmic function σ factor (σ^ECF) in the cytosol in response to an extracellular signal. Activation requires the regulated and sequential proteolysis of the σ^ECF-associated anti-σ factor, and the function of the Prc and RseP proteases. In this work, we have identified another protease that modulates CSS activity, namely the periplasmic carboxyl-terminal processing protease CtpA. CtpA functions upstream of Prc in the proteolytic cascade and seems to prevent the Prc-mediated proteolysis of the CSS anti-σ factor. Importantly, using zebrafish embryos and the A549 lung epithelial cell line as hosts, we show that mutants in the rseP and ctp

SUBMITTER: Otero-Asman JR 

PROVIDER: S-EPMC10392083 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature