Transformation of aqueous protein attenuated total reflectance infra-red absorbance spectroscopy to transmission.

ABSTRACT: Infrared (IR) spectroscopy is increasingly being used to probe the secondary structure of proteins, especially for high-concentration samples and biopharmaceuticals in complex formulation vehicles. However, the small path lengths required for aqueous protein transmission experiments, due to high water absorbance in the amide I region of the spectrum, means that the path length is not accurately known, so only the shape of the band is ever considered. This throws away a dimension of information. Attenuated total reflectance (ATR) IR spectroscopy is much easier to implement than transmission IR spectroscopy and, for a given instrument and sample, gives reproducible spectra. However, the ATR-absorbance spectrum varies with sample concentration and instrument configuration, and its wavenumber

SUBMITTER: Rodger A 

PROVIDER: S-EPMC10392695 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature