Glucose promoting the early embryonic development by increasing the lipid synthesis at 2-cell stage.

ABSTRACT: The optimization of culture conditions is one of the main strategies to improve the embryo development competence in in vitro fertilization (IVF). Glucose is an important carbon source while also exists in the oviductal fluid in vivo, the effect of glucose in embryo development microenvironment is still unclear. Here we employed the LC-MS to detect and analyze the metabolites in the culture medium of different cleavage stages including 2-Cell, 4-Cell and 8-Cell mouse embryos, respectively. The effects of the external glucose were estimated by measuring the development rate at different glucose concentrations from 0 to 5 mmol/L, and the gene expression changes were detected to explore the potential mechanism after the addition of glucose in the media. Our results indicated the

SUBMITTER: Wang M 

PROVIDER: S-EPMC10392834 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature