Airway and Systemic Prostaglandin E2 Association with COPD Symptoms and Macrophage Phenotype.

ABSTRACT:

Background

Polymorphisms and products of the cyclooxygenase (COX) pathway have been associated with the development of chronic obstructive pulmonary disease (COPD) and adverse outcomes. COX-produced prostaglandin E2 (PGE-2) may play a role in the inflammation observed in COPD, potentially through deleterious airway macrophage polarization. A better understanding of the role of PGE-2 in COPD morbidity may inform trials for therapeutics targeting the COX pathway or PGE-2.

Methods

Urine and induced sputum were collected from former smokers with moderate-severe COPD. The major urinary metabolite of PGE-2 (PGE-M) was measured, and ELISA was performed on sputum supernatant for PGE-2 airway measurement. Airway macrophages underwent flow cytometry phenotyping (surface CD64, CD80, CD

SUBMITTER: Tejwani V 

PROVIDER: S-EPMC10392871 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature