In Vitro Transcription-Translation in an Artificial Biomolecular Condensate.

ABSTRACT: Biomolecular condensates are a promising platform for synthetic cell formation and constitute a potential missing link between the chemical and cellular stage of the origins of life. However, it has proven challenging to integrate complex reaction networks into biomolecular condensates, such as a cell-free in vitro transcription-translation (IVTT) system. Integrating IVTT into biomolecular condensates successfully is one precondition for condensation-based synthetic cell formation. Moreover, it would provide a proof of concept that biomolecular condensates are in principle compatible with the central dogma, one of the hallmarks of cellular life. Here, we have systemically investigated the compatibility of eight different (bio)molecular condensates with IVTT incorporation. Of these eight ca

SUBMITTER: Schoenmakers LLJ 

PROVIDER: S-EPMC10393115 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature