Nicotinamide enhances osteoblast differentiation through activation of the mitochondrial antioxidant defense system.

ABSTRACT: Although the normal physiological level of oxidative stress is beneficial for maintaining bone homeostasis, imbalance between reactive oxygen species (ROS) production and antioxidant defense can cause various bone diseases. The purpose of this study was to determine whether nicotinamide (NAM), an NAD⁺ precursor, can support the maintenance of bone homeostasis by regulating osteoblasts. Here, we found that NAM enhances osteoblast differentiation and mitochondrial metabolism. NAM increases the expression of antioxidant enzymes, which is due to increased FOXO3A transcriptional activity via SIRT3 activation. NAM has not only a preventive effect against weak and chronic oxidative stress but also a therapeutic effect against strong and acute exposure to H₂O₂ in o

SUBMITTER: Yoon H 

PROVIDER: S-EPMC10393969 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature