Protocol for isolation of mouse pre-implantation embryos for gene expression analysis.

ABSTRACT: Visualizing and quantifying the numerous factors that regulate murine pre-implantation embryonic development is technically challenging. Here, we present a protocol for the isolation of pre-implantation embryos at multiple stages of embryonic development to study gene expression. We describe steps for isolating RNA and cDNA synthesis from a small number of embryos. We then detail an immunofluorescence assay for the detection and localization of protein of interest by confocal microscopy in the pre-implantation embryos. For complete details on the use and execution of this protocol, please refer to Varghese et al.¹.

SUBMITTER: Varghese PC 

PROVIDER: S-EPMC10394003 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature