Splicing factor TRA2A contributes to esophageal cancer progression via a noncanonical role in lncRNA m⁶ A methylation.

ABSTRACT: Transformer 2 alpha homolog (TRA2A), a member of the serine/arginine-rich splicing factor family, has been shown to control mRNA splicing in development and cancers. However, it remains unclear whether TRA2A is involved in lncRNA regulation. In the present study, we found that TRA2A was upregulated and correlated with poor prognosis in esophageal cancer. Downregulation of TRA2A suppressed the tumor growth in xenograft nude mice. Epitranscriptomic microarray showed that depletion of TRA2A affected global lncRNA methylation similarly to the key m⁶ A methyltransferase, METTL3, by silencing. MeRIP-qPCR, RNA pull-down, CLIP analyses, and stability assays indicated that ablation of TRA2A reduced m⁶ A-modification of the oncogenic lncRNA MALAT1, thus inducing structural alte

SUBMITTER: Bei M 

PROVIDER: S-EPMC10394134 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature