Multimodal immune cell phenotyping in GI biopsies reveals microbiome-related T cell modulations in human GvHD.

ABSTRACT: Acute graft-versus-host disease (aGvHD) is a significant complication after allogeneic hematopoietic stem cell transplantation (aHSCT), but major factors determining disease severity are not well defined yet. By combining multiplexed tissue imaging and single-cell RNA sequencing on gastrointestinal biopsies from aHSCT-treated individuals with fecal microbiome analysis, we link high microbiome diversity and the abundance of short-chain fatty acid-producing bacteria to the sustenance of suppressive regulatory T cells (Tregs). Furthermore, aGvHD severity strongly associates with the clonal expansion of mainly CD8 T cells, which we find distributed over anatomically distant regions of the gut, persistent over time, and inversely correlated with the presence of suppressive Tregs. Overall, our s

SUBMITTER: Jarosch S 

PROVIDER: S-EPMC10394271 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature