Plasma extracellular vesicle test sample to standardize flow cytometry measurements.

ABSTRACT:

Background

Extracellular vesicles (EVs) in body fluids are explored as disease biomarkers, but EV concentrations measured by flow cytometers (FCMs) are incomparable.

Objectives

To improve data comparability, new reference materials with physical properties resembling EVs and reference procedures are being developed. The validation of new reference materials and procedures requires biological test samples. We developed a human plasma EV test sample (PEVTES) that i) resembles subcellular particles in plasma, ii) is ready-to-use, iii) is flow cytometry-compatible, and iv) is stable.

Methods

The PEVTES was prepared from human plasma of 3 fasting donors. EVs were immunofluorescently stained with antibodies against platelet-specific (CD61) and erythrocyte-specific (CD235a)

SUBMITTER: Bettin B 

PROVIDER: S-EPMC10394550 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature