Regulation of megakaryo/thrombopoiesis by endosomal toll-like receptor 7 and 8 activation of CD34⁺ cells in a viral infection model.

ABSTRACT:

Background

CD34⁺ cells, megakaryocytes (MKs), and platelets express toll-like receptors (TLRs) that enable these cells to amplify the host innate immune response. However, the role of TLR7/TLR8 activation in megakaryopoiesis has not yet been investigated.

Objectives

We evaluated the effect of coxsackievirus B3 (CVB3) and synthetic TLR7/TLR8 agonists on the development of human MKs and production of platelets.

Methods

CD34⁺ cells from human umbilical cord were inoculated with CVB3 or stimulated with synthetic TLR7/TLR8 agonists and then cultured in the presence of thrombopoietin.

Results

CD34⁺ cells, MK progenitor cells, and mature MKs expressed TLR7 and TLR8, and exposure to CVB3 resulted in productive infection, as determined b

SUBMITTER: Rodriguez CS 

PROVIDER: S-EPMC10394566 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature