The retinoic acid response is a minor component of the cardiac phenotype in H9c2 myoblast differentiation.

ABSTRACT: The H9c2 myoblast cell line, isolated from the left ventricular tissue of rat, is currently used in vitro as a mimetic for skeletal and cardiac muscle due to its biochemical, morphological, and electrical/hormonal signaling properties. During culture, H9c2 cells acquire a myotube phenotype, where a critical component is the inclusion of retinoic acid (RA). The results from some authors on H9c2 suggested that thousands of genes respond to RA stimuli, while others report hundreds of genes responding to RA over different cell types. In this article, using a more appropriate experimental design, we first confirm the H9c2 cardiac phenotype with and without RA and report transcriptomic and physiological changes regarding calcium handling, bioenergetics, and other biological concepts. Interesting

SUBMITTER: Campero-Basaldua C 

PROVIDER: S-EPMC10394869 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature