Asiaticoside ameliorates renal ischemia/reperfusion injury by promoting CD4⁺CD25⁺FOXP3⁺ treg cell differentiation.

ABSTRACT: Ischemia/reperfusion injury (I/R) is the major cause of acute kidney injury, which becomes a global health problem. The effects of asiaticoside, as an anti-inflammatory drug, on renal ischemia-reperfusion injury have not been well defined. After the CD4⁺ cells were treated with asiaticoside, the CD4⁺CD25+FOXP3+ Treg cell differentiation was detected by flow cytometry. The viability and release of inflammatory factors of CD4⁺CD25+FOXP3+ Treg cell were detected by CCK-8 and ELISA. Renal I/R injury mice model was established, and the mice were pre-treated with asiaticoside or CD25 antibody or infused with Treg cells. The histological changes of renal tissue were evaluated by Hematoxylin-eosin, PAS, and Masson staining. The renal function markers were evaluated

SUBMITTER: Tang S 

PROVIDER: S-EPMC10395025 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature