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A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells.

ABSTRACT: Calcium is a ubiquitous second messenger in eukaryotes, correlated with neuronal activity and T-cell activation among other processes. Real-time calcium indicators such as GCaMP have recently been complemented by newer calcium integrators that convert transient calcium activity into stable gene expression. Here we introduce LuCID, a dual-purpose real-time calcium indicator and transcriptional calcium integrator that combines the benefits of both calcium detection technologies. We show that the calcium-dependent split luciferase component of LuCID provides a real-time bioluminescence readout of calcium dynamics in cells, while the GI/FKF1 split GAL4 component of LuCID converts calcium-generated bioluminescence into stable gene expression. We also show that LuCID's modular design enables it to read out other cellular events such as protein-protein interactions. LuCID adds to the arsenal of tools for studying cells and cell populations that utilize calcium for signaling.

SUBMITTER: Erdenee E

PROVIDER: S-EPMC10395047 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells.

Erdenee Elbegduuren E Ting Alice Y AY

ACS synthetic biology 20220307 3

Calcium is a ubiquitous second messenger in eukaryotes, correlated with neuronal activity and T-cell activation among other processes. Real-time calcium indicators such as GCaMP have recently been complemented by newer calcium integrators that convert transient calcium activity into stable gene expression. Here we introduce LuCID, a dual-purpose real-time calcium indicator and transcriptional calcium integrator that combines the benefits of both calcium detection technologies. We show that the c ...[more]

PMID: 35254056

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