The phospholipase effector Tle1^Vc promotes Vibrio cholerae virulence by killing competitors and impacting gene expression.

ABSTRACT: Vibrio cholerae utilizes the Type VI secretion system (T6SS) to gain an advantage in interbacterial competition by delivering anti-prokaryotic effectors in a contact-dependent manner. However, the impact of T6SS and its secreted effectors on physiological behavior remains poorly understood. In this study, we present Tle1^Vc, a phospholipase effector in atypical pathogenic V. cholerae E1 that is secreted by T6SS via its interaction with VgrG1^E1. Tle1^Vc contains a DUF2235 domain and belongs to the Tle1 (type VI lipase effector) family. Bacterial toxicity assays, lipase activity assays and site-directed mutagenesis revealed that Tle1^Vc possessed phospholipase A₁ activity and phospholipase A₂ activity, and that Tle1

SUBMITTER: Liu M 

PROVIDER: S-EPMC10395195 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature