Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication.

ABSTRACT: The genomes of positive-sense RNA viruses encode polyproteins that are essential for mediating viral replication. These viral polyproteins must undergo proteolysis (also termed polyprotein processing) to generate functional protein units. This proteolysis can be performed by virally-encoded proteases as well as host cellular proteases, and is generally believed to be a key step in regulating viral replication. Hepatitis E virus (HEV) is a leading cause of acute viral hepatitis. The positive-sense RNA genome is translated to generate a polyprotein, termed pORF1, which is necessary and sufficient for viral genome replication. However, the mechanism of polyprotein processing in HEV remains to be determined. In this study, we aimed to understand processing of this polyprotein and its role in v

SUBMITTER: Pierce DM 

PROVIDER: S-EPMC10395923 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature