Critical role of the RpoE stress response pathway in polymyxin resistance of Escherichia coli.

ABSTRACT:

Objectives

Polymyxins, including colistin, are the drugs of last resort to treat MDR bacterial infections in humans. In-depth understanding of the molecular basis and regulation of polymyxin resistance would provide new therapeutic opportunities to combat increasing polymyxin resistance. Here we aimed to identify novel targets that are crucial for polymyxin resistance using Escherichia coli BL21(DE3), a unique colistin-resistant model strain.

Methods

BL21(DE3) was subjected to random transposon mutagenesis for screening colistin-susceptible mutants. The insertion sites of desired mutants were mapped; the key genes of interest were also inactivated in different strains to examine functional conservation. Specific genes in the known PmrAB and PhoPQ regulatory network were inac

SUBMITTER: Zeng X 

PROVIDER: S-EPMC10396327 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature