Mutational analysis of the avian adenovirus CELO, which provides a basis for gene delivery vectors.

ABSTRACT: The avian adenovirus CELO is being developed as a gene transfer tool. Using homologous recombination in Escherichia coli, the CELO genome was screened for regions that could be deleted and would tolerate the insertion of a marker gene (luciferase or enhanced green fluorescent protein). For each mutant genome, the production of viable virus able to deliver the transgene to target cells was monitored. A series of mutants in the genome identified a set of open reading frames that could be deleted but which must be supplied in trans for virus replication. A region of the genome which is dispensable for viral replication and allows the insertion of an expression cassette was identified and a vector based on this mutation was evaluated as a gene delivery reagent. Transduction of avian cells occu

SUBMITTER: Michou AI 

PROVIDER: S-EPMC103964 | biostudies-literature | 1999 Feb

REPOSITORIES: biostudies-literature