Deterministic early endosomal maturations emerge from a stochastic trigger-and-convert mechanism.

ABSTRACT: Endosomal maturation is critical for robust and timely cargo transport to specific cellular compartments. The most prominent model of early endosomal maturation involves a phosphoinositide-driven gain or loss of specific proteins on individual endosomes, emphasising an autonomous and stochastic description. However, limitations in fast, volumetric imaging long hindered direct whole cell-level measurements of absolute numbers of maturation events. Here, we use lattice light-sheet imaging and bespoke automated analysis to track individual very early (APPL1-positive) and early (EEA1-positive) endosomes over the entire population, demonstrating that direct inter-endosomal contact drives maturation between these populations. Using fluorescence lifetime, we show that this endosomal interaction i

SUBMITTER: York HM 

PROVIDER: S-EPMC10397212 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature