Proteogenomic analysis reveals RNA as a source for tumor-agnostic neoantigen identification.

ABSTRACT: Systemic pan-tumor analyses may reveal the significance of common features implicated in cancer immunogenicity and patient survival. Here, we provide a comprehensive multi-omics data set for 32 patients across 25 tumor types for proteogenomic-based discovery of neoantigens. By using an optimized computational approach, we discover a large number of tumor-specific and tumor-associated antigens. To create a pipeline for the identification of neoantigens in our cohort, we combine DNA and RNA sequencing with MS-based immunopeptidomics of tumor specimens, followed by the assessment of their immunogenicity and an in-depth validation process. We detect a broad variety of non-canonical HLA-binding peptides in the majority of patients demonstrating partially immunogenicity. Our validation process a

SUBMITTER: Tretter C 

PROVIDER: S-EPMC10397250 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature