Immune landscape in rejection of renal transplantation revealed by high-throughput single-cell RNA sequencing.

ABSTRACT: Background: The role of the cellular level in kidney transplant rejection is unclear, and single-cell RNA sequencing (scRNA-seq) can reveal the single-cell landscape behind rejection of human kidney allografts at the single-cell level. Methods: High-quality transcriptomes were generated from scRNA-seq data from five human kidney transplantation biopsy cores. Cluster analysis was performed on the scRNA-seq data by known cell marker genes in order to identify different cell types. In addition, pathways, pseudotime developmental trajectories and transcriptional regulatory networks involved in different cell subpopulations were explored. Next, we systematically analyzed the scoring of gene sets regarding single-cell expression profiles based on biological processes associated wit

SUBMITTER: Wen N 

PROVIDER: S-EPMC10397399 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature