Oxidative damage from repeated tissue isolation for subculturing causes degeneration in Volvariella volvacea.

ABSTRACT: The fungal fruiting body is the organized mycelium. Tissue isolation and mycelium succession are common methods of fungal species purification and rejuvenation in the production of edible mushrooms. However, repeated succession increases strain degeneration. In this study, we examined the effect of repeated tissue isolation from Volvariella volvacea fruitbodies on the occurrence of degeneration. The results showed that less than four times in succession improved production capacity, however, after 12 successions, the traits indicating strain degeneration were apparent. For instance, the density of aerophytic hyphae, hyphal growth rate and hyphal biomass were gradually reduced, while the hyphae branching was increased. Also, other degenerative traits such as prolonged production cycl

SUBMITTER: Zhao F 

PROVIDER: S-EPMC10397519 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature