Surfactants or scaffolds? RNAs of varying lengths control the thermodynamic stability of condensates differently.

ABSTRACT: Biomolecular condensates, thought to form via liquid-liquid phase separation of intracellular mixtures, are multicomponent systems that can include diverse types of proteins and RNAs. RNA is a critical modulator of RNA-protein condensate stability, as it induces an RNA concentration-dependent reentrant phase transition-increasing stability at low RNA concentrations and decreasing it at high concentrations. Beyond concentration, RNAs inside condensates can be heterogeneous in length, sequence, and structure. Here, we use multiscale simulations to understand how different RNA parameters interact with one another to modulate the properties of RNA-protein condensates. To do so, we perform residue/nucleotide resolution coarse-grained molecular dynamics simulations of multicomponent RNA-protein

SUBMITTER: Sanchez-Burgos I 

PROVIDER: S-EPMC10398262 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature