Siderocalin fusion proteins enable a new ⁸⁶Y/⁹⁰Y theranostic approach.

ABSTRACT: The mammalian protein siderocalin binds bacterial siderophores and their iron complexes through cation-π and electrostatic interactions, but also displays high affinity for hydroxypyridinone complexes of trivalent lanthanides and actinides. In order to circumvent synthetic challenges, the use of siderocalin-antibody fusion proteins is explored herein as an alternative targeting approach for precision delivery of trivalent radiometals. We demonstrate the viability of this approach in vivo, using the theranostic pair ⁹⁰Y (β^-, t_1/2 = 64 h)/⁸⁶Y (β⁺, t_1/2 = 14.7 h) in a SKOV-3 xenograft mouse model. Ligand radiolabeling with octadentate hydroxypyridinonate 3,4,3-LI(1,2-HOPO) and subsequent protein binding wer

SUBMITTER: Cosby AG 

PROVIDER: S-EPMC10398355 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature