Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration.

ABSTRACT: Increased understanding of plant genetics and the development of powerful and easier-to-use gene editing tools over the past century have revolutionized humankind's ability to deliver precise genotypes in crops. Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. Typical protocols to make gene edited (GE) varieties also use transgenes, even though these may be undesirable in the final crop product. In some crops, the transgenes are routinely se

SUBMITTER: Kocsisova Z 

PROVIDER: S-EPMC10398581 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature