Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy.

ABSTRACT: Cells can crawl, self-heal, and tune their stiffness due to their remarkably dynamic cytoskeleton. As such, reconstituting networks of cytoskeletal biopolymers may lead to a host of active and adaptable materials. However, engineering such materials with precisely tuned properties requires measuring how the dynamics depend on the network composition and synthesis methods. Quantifying such dynamics is challenged by variations across the time, space, and formulation space of composite networks. The protocol here describes how the Fourier analysis technique, differential dynamic microscopy (DDM), can quantify the dynamics of biopolymer networks and is particularly well suited for studies of cytoskeleton networks. DDM works on time sequences of images acquired using a range of microscopy modal

SUBMITTER: Verwei HN 

PROVIDER: S-EPMC10398790 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature