Serotonin 5-HT₇ receptor slows down the G_s protein: a single molecule perspective.

ABSTRACT: The 5-hydroxytryptamine (serotonin) receptor type 7 (5-HT₇R) is a G protein-coupled receptor present primarily in the nervous system and gastrointestinal tract, where it regulates mood, cognition, digestion, and vasoconstriction. 5-HT₇R has previously been shown to bind to its cognate stimulatory G_s protein in the inactive state. This phenomenon, termed "inverse coupling," is thought to counteract the atypically high intrinsic activity of 5-HT₇R. However, it is not clear how active and inactive 5-HT₇ receptors affect the mobility of the G_s protein in the plasma membrane. Here, we used single-molecule imaging of the G_s protein and 5-HT₇R to evaluate G_s mobility in the membrane in the presence

SUBMITTER: Petelak A 

PROVIDER: S-EPMC10398887 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature