GSG2 facilitates the progression of human breast cancer through MDM2-mediated ubiquitination of E2F1.

ABSTRACT:

Background

Breast cancer (BC) has posed a great threat to world health as the leading cause of cancer death among women. Previous evidence demonstrated that germ cell-specific gene 2 (GSG2) was involved in the regulation of multiple cancers. Thus, the clinical value, biological function and underlying mechanism of GSG2 in BC were investigated in this study.

Methods

The expression of GSG2 in BC was revealed by immunohistochemistry (IHC), qPCR and western blotting. Secondly, the biological function of GSG2 in BC was evaluated by MTT assay, flow cytometry, Transwell assay and wound healing assay. Furthermore, the potential molecular mechanism of GSG2 regulating the progression of BC by co-immunoprecipitation (Co-IP) and protein stability detection.

Results

Our data indic

SUBMITTER: Tang Y 

PROVIDER: S-EPMC10398932 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature