Differential effects of SARS-CoV-2 variants on central nervous system cells and blood-brain barrier functions.

ABSTRACT:

Background

Although mainly causing a respiratory syndrome, numerous neurological symptoms have been identified following of SARS-CoV-2 infection. However, how the virus affects the brain and how the mutations carried by the different variants modulate those neurological symptoms remain unclear.

Methods

We used primary human pericytes, foetal astrocytes, endothelial cells and a microglial cell line to investigate the effect of several SARS-CoV-2 variants of concern or interest on their functional activities. Cells and a 3D blood-brain barrier model were infected with the wild-type form of SARS-CoV-2, Alpha, Beta, Delta, Eta, or Omicron (BA.1) variants at various MOI. Cells and supernatant were used to evaluate cell susceptibility to the virus using a microscopic assay as well

SUBMITTER: Proust A 

PROVIDER: S-EPMC10398935 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature