Cooperative cell-cell actin network remodeling to perform Gap junction endocytosis.

ABSTRACT:

Background

The endocytosis of Gap junction plaques (GJP) requires cytoskeletal forces to internalize such large membranous structures. Actin, which partners the connexin proteins constituting Gap junctions and is located close to Annular Gap Junctions (AGJ), could be actively involved in this physiological process.

Results

Electron Microscopy and Light Microscopy images, associated with time-lapse analysis and 3D reconstruction, used at high resolution and enhanced using ImageJ based software analysis, revealed that: i) actin cables, originating from Donor cells, insert on the edge of GJP and contribute to their invagination, giving rise to AGJ, whereas actin cables on the Acceptor cell side of the plaque are not modified; ii) actin cables from the Donor cell are continuous

SUBMITTER: Segretain D 

PROVIDER: S-EPMC10399049 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature