Molecular and Dual-Isotopic Profiling of the Microbial Controls on Nitrogen Leaching in Agricultural Soils under Managed Aquifer Recharge.

ABSTRACT: Nitrate (NO₃^-) leaching is a serious health and ecological concern in global agroecosystems, particularly those under the application of agricultural-managed aquifer recharge (Ag-MAR); however, there is an absence of information on microbial controls affecting NO₃^- leaching outcomes. We combine natural dual isotopes of NO₃^- (¹⁵N/¹⁴N and ¹⁸O/¹⁶O) with metagenomics, quantitative polymerase chain reaction (PCR), and a threshold indicator taxa analysis (TITAN) to investigate the activities, taxon profiles, and environmental controls of soil microbiome associated with NO₃^- leaching at different depths from Californian vineyards under Ag-MAR application. The isotopi

SUBMITTER: Huang L 

PROVIDER: S-EPMC10399200 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature