Visualization of Polyhydroxyalkanoate Accumulated in Waste Activated Sludge.

ABSTRACT: Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge microbial communities. Selective staining methods were applied to a municipal activated sludge during pilot scale PHA accumulation in replicate experiments. Visualization of stained flocs revealed that a significant but limited fraction of the biomass was engaged with PHA accumulation. Accumulated PHA granules were furthermore heterogeneously distributed within and between flocs. These observations suggested that the PHA content for the bacteria storing P

SUBMITTER: Pei R 

PROVIDER: S-EPMC10399201 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature