Fast and automated protein-DNA/RNA macromolecular complex modeling from cryo-EM maps.

ABSTRACT: Cryo-electron microscopy (cryo-EM) allows a macromolecular structure such as protein-DNA/RNA complexes to be reconstructed in a three-dimensional coulomb potential map. The structural information of these macromolecular complexes forms the foundation for understanding the molecular mechanism including many human diseases. However, the model building of large macromolecular complexes is often difficult and time-consuming. We recently developed DeepTracer-2.0, an artificial-intelligence-based pipeline that can build amino acid and nucleic acid backbones from a single cryo-EM map, and even predict the best-fitting residues according to the density of side chains. The experiments showed improved accuracy and efficiency when benchmarking the performance on independent experimental maps of prote

SUBMITTER: Nakamura A 

PROVIDER: S-EPMC10399284 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature