Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.

ABSTRACT: Plastids are complex organelles that vary in size and function depending on the cell type. Accordingly, they can be referred to as amyloplasts, chloroplasts, chromoplasts, etioplasts, or proplasts, to only cite a few. Over the past decades, methods based on density gradients and differential centrifugation have been extensively used for the purification of plastids. However, these methods need large amounts of starting material, and hardly provide a tissue-specific resolution. Here, we applied our IPTACT (Isolation of Plastids TAgged in specific Cell Types) method, which involves the biotinylation of plastids in vivo using one-shot transgenic lines expressing the Translocon of the Outer Membrane 64 (TOC64) gene coupled with a biotin ligase receptor particle and the BirA biotin ligase, to i

SUBMITTER: Boussardon C 

PROVIDER: S-EPMC10400147 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature