Network-wide thermodynamic constraints shape NAD(P)H cofactor specificity of biochemical reactions.

ABSTRACT: The ubiquitous coexistence of the redox cofactors NADH and NADPH is widely considered to facilitate an efficient operation of cellular redox metabolism. However, it remains unclear what shapes the NAD(P)H specificity of specific redox reactions. Here, we present a computational framework to analyze the effect of redox cofactor swaps on the maximal thermodynamic potential of a metabolic network and use it to investigate key aspects of redox cofactor redundancy in Escherichia coli. As one major result, our analysis suggests that evolved NAD(P)H specificities are largely shaped by metabolic network structure and associated thermodynamic constraints enabling thermodynamic driving forces that are close or even identical to the theoretical optimum and significantly higher compared to random spec

SUBMITTER: Bekiaris PS 

PROVIDER: S-EPMC10400544 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature