Immunoprecipitation methods impact the peptide repertoire in immunopeptidomics.

ABSTRACT:

Introduction

Mass spectrometry-based immunopeptidomics is the only unbiased method to identify naturally presented HLA ligands, which is an indispensable prerequisite for characterizing novel tumor antigens for immunotherapeutic approaches. In recent years, improvements based on devices and methodology have been made to optimize sensitivity and throughput in immunopeptidomics. However, developments in ligand isolation, mass spectrometric analysis, and subsequent data processing can have a marked impact on the quality and quantity of immunopeptidomics data.

Methods

In this work, we compared the immunopeptidome composition in terms of peptide yields, spectra quality, hydrophobicity, retention time, and immunogenicity of two established immunoprecipitation methods (column-based

SUBMITTER: Wacker M 

PROVIDER: S-EPMC10400765 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature