The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin.

ABSTRACT:

Background

Chemoresistance is a common event after cancer chemotherapy, including gastric cancer (GC). Cisplatin has been reported to induce the DNA damage response (DDR), thus leading to chemoresistance. VE-821, a specific inhibitor of ATR, has been proven to suppress a variety of solid malignancies effectively. Our study aimed to explore the effect of VE-821 on enhancing the chemical sensitivity to cisplatin and clarify the potential molecular mechanisms.

Methods

Cell viability and apoptosis of MKN-45 and AGS were measured by CCK8 and flow cytometry assay respectively. Western blotting was used to detect the expression of target proteins. TCGA database was used to analyze the correlation between the ATR expression with the prognosis of GC patients. The viability of GC orga

SUBMITTER: Su H 

PROVIDER: S-EPMC10400920 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature