Ontology highlight
ABSTRACT:
SUBMITTER: Feldmann C
PROVIDER: S-EPMC10400964 | biostudies-literature | 2023 Jul
REPOSITORIES: biostudies-literature
Feldmann Colin C Schänzler Michael M Ben-Johny Manu M Wahl-Schott Christian C
STAR protocols 20230728 3
Two-hybrid Förster resonance energy transfer (FRET) provides proximity, affinity, and stoichiometry information in binding interactions. We present an image-based approach that surpasses traditional two-hybrid FRET assays in precision and robustness. We outline instrument setup and image acquisition and further describe steps for image preprocessing and two-hybrid FRET analysis using provided software to simplify the workflow. This protocol is compatible with confocal microscopes for high-precis ...[more]