Surface-induced phase separation of reconstituted nascent integrin clusters on lipid membranes.

ABSTRACT: Integrin adhesion complexes are essential membrane-associated cellular compartments for metazoan life. The formation of initial integrin adhesion complexes is a dynamic process involving focal adhesion proteins assembled at the integrin cytoplasmic tails and the inner leaflet of the plasma membrane. The weak multivalent protein interactions within the complex and with the plasma membrane suggest that liquid-liquid phase separation could play a role in the nascent adhesion assembly. Here, we report that solid-supported lipid membranes supplemented with phosphoinositides induce the phase separation of minimal integrin adhesion condensates composed of integrin β1 tails, kindlin, talin, paxillin, and FAK at physiological ionic strengths and protein concentrations. We show that the prese

SUBMITTER: Hsu CP 

PROVIDER: S-EPMC10400992 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature