Vasodilators mobilize SK3 channels in endothelial cells to produce arterial relaxation.

ABSTRACT: Endothelial cells (ECs) line the lumen of all blood vessels and regulate functions, including contractility. Physiological stimuli, such as acetylcholine (ACh) and intravascular flow, activate transient receptor potential vanilloid 4 (TRPV4) channels, which stimulate small (SK3)- and intermediate (IK)-conductance Ca²⁺-activated potassium channels in ECs to produce vasodilation. Whether physiological vasodilators also modulate the surface abundance of these ion channels in ECs to elicit functional responses is unclear. Here, we show that ACh and intravascular flow stimulate rapid anterograde trafficking of an intracellular pool of SK3 channels in ECs of resistance-size arteries, which increases surface SK3 protein more than two-fold. In contrast, ACh and flow do not alter the sur

SUBMITTER: Peixoto-Neves D 

PROVIDER: S-EPMC10401010 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature