G9a promotes immune suppression by targeting the Fbxw7/Notch pathway in glioma stem cells.

ABSTRACT:

Aim

Immunotherapy for glioblastoma multiforme (GBM) is limited because of a strongly immunosuppressive tumor microenvironment (TME). Remodeling the immune TME is an effective strategy to eliminate GBM immunotherapy resistance. Glioma stem cells (GSCs) are inherently resistant to chemotherapy and radiotherapy and involved in immune evasion mechanism. This study aimed to investigate the effects of histone methyltransferases 2 (EHMT2 or G9a) on immunosuppressive TME and whether this effect was related to changes on cell stemness.

Methods

Tumor-infiltrating immune cells were analyzed by flow cytometry and immunohistochemistry in orthotopic implanted glioma mice model. The gene expressions were measured by RT-qPCR, western blot, immunofluorescence, and flow cytometry. Cell viabil

SUBMITTER: Cao Y 

PROVIDER: S-EPMC10401078 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature