Collagen Fibril Orientation Instructs Fibroblast Differentiation Via Cell Contractility.

ABSTRACT: Collagen alignment is one of the key microarchitectural signatures of many pathological conditions, including scarring and fibrosis. Investigating how collagen alignment modulates cellular functions will pave the way for understanding tissue scarring and regeneration and new therapeutic strategies. However, current approaches for the fabrication of three-dimensional (3D) aligned collagen matrices are low-throughput and require special devices. To overcome these limitations, a simple approach to reconstitute homogeneous 3D collagen matrices with adjustable degree of fibril alignment using 3D printed inclined surfaces is developed. By characterizing the mechanical properties of reconstituted matrices, it is found that the elastic modulus of collagen matrices is enhanced with an increase in t

SUBMITTER: Sapudom J 

PROVIDER: S-EPMC10401101 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature