Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway.

ABSTRACT:

Background

This study aimed to investigate the potential mechanism of paeonol in the treatment of neuropathic pain.

Methods

Relevant mechanisms were explored through microglial pseudotime analysis and the use of specific inhibitors in cell experiments. In animal experiments, 32 SD rats were randomly divided into the sham operation group, the chronic constrictive injury (CCI) group, the ibuprofen group, and the paeonol group. We performed behavioral testing, ELISA, PCR, Western blotting, immunohistochemistry, and immunofluorescence analysis.

Results

The pseudotime analysis of microglia found that RhoA, Rock1, and p38MAPK were highly expressed in activated microglia, and the expression patterns of these genes were consistent with the expression trends of the M1 markers

SUBMITTER: Li X 

PROVIDER: S-EPMC10401133 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature