Cigarette smoke stimulates clonal expansion of Jak2^V617F and Tet2^-/- cells.

ABSTRACT:

Introduction

Somatic mutations in myeloid growth factor pathway genes, such as JAK2, and genes involved in epigenetic regulation, such as TET2, in hematopoietic stem cells (HSCs) leads to clonal hematopoiesis of indeterminate potential (CHIP) which presents a risk factor for hematologic malignancy and cardiovascular disease. Smoking behavior has been repeatedly associated with the occurrence of CHIP but whether smoking is an environmental inflammatory stressor in promoting clonal expansion has not been investigated.

Methods

We performed in vivo smoke exposures in both wildtype (WT) mice and transplanted mice carrying Jak2^V617F mutant and Tet2 knockout (Tet-/-) cells to determine the impact of cigarette smoke (CS) in the HSC compartment as well as favoring mutant c

SUBMITTER: Ramanathan G 

PROVIDER: S-EPMC10401270 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature