Intratumoral NKp46⁺ natural killer cells are spatially distanced from T and MHC-I⁺ cells with prognostic implications in soft tissue sarcoma.

ABSTRACT:

Introduction

Soft tissue sarcomas (STS) are rare, heterogenous malignancies with an unmet need for novel immunotherapies. Tumor infiltrating lymphocytes (TILs) have been linked with favorable outcomes in STS patients, though the contribution of natural killer (NK) cells and spatial relationships of TILs with MHC-I expressing cells lacks detailed characterization.

Experimental design

Using archived and prospectively collected specimens, we evaluated intratumoral NK cells by immunohistochemistry (IHC), flow cytometry, and immunofluorescence (IF). We assessed spatial localization of NK and T cells by multiplex IF, analyzing the effects of MHC-I expression status on NK and T cell clustering.

Results

Both intratumoral NKp46 and CD56^dim expression were associated

SUBMITTER: Cruz SM 

PROVIDER: S-EPMC10401426 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature