Multicomponent Oxidative Nitrile Thiazolidination Reaction for Selective Modification of N-terminal Dimethylation Posttranslational Modification.

ABSTRACT: Protein α-N-terminal dimethylation (Nme₂) is an underexplored posttranslational modification (PTM) despite the increasing implications of α-N-terminal dimethylation in vital physiological and pathological processes across diverse species; thus, it is imperative to identify the sites of α-N-terminal dimethylation in the proteome. So far, only ∼300 α-N-terminal methylation sites have been discovered including mono-, di-, and tri-methylation, due to the lack of a pan-selective method for detecting α-N-terminal dimethylation. Herein, we introduce the three-component coupling reaction, oxidative nitrile thiazolidination (OxNiTha) for chemoselective modification of α-Nme₂ to thiazolidine ring in the presence of selectfluor, sodium cyanide, and 1,2 aminothiols. One of the ma

SUBMITTER: Emenike B 

PROVIDER: S-EPMC10401698 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature