Leveraging Steric Moieties for Kinetic Control of DNA Strand Displacement Reactions.

ABSTRACT: DNA strand displacement networks are a critical part of dynamic DNA nanotechnology and are proven primitives for implementing chemical reaction networks. Precise kinetic control of these networks is important for their use in a range of applications. Among the better understood and widely leveraged kinetic properties of these networks are toehold sequence, length, composition, and location. While steric hindrance has been recognized as an important factor in such systems, a clear understanding of its impact and role is lacking. Here, a systematic investigation of steric hindrance within a DNA toehold-mediated strand displacement network was performed through tracking kinetic reactions of reporter complexes with incremental concatenation of steric moieties near the toehold. Two subsets of s

SUBMITTER: Lysne D 

PROVIDER: S-EPMC10401717 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature