Low androgen levels induce ferroptosis of rat penile cavernous endothelial cells.

ABSTRACT:

Background

Endothelial dysfunction caused by low androgen levels in penile tissue can lead to erectile dysfunction. The exact mechanism of endothelial dysfunction has not been thoroughly studied.

Objective

The study sought to verify whether low androgen levels induce ferroptosis of endothelial cells in rat penile tissue.

Methods

Rat penile cavernous endothelial cells (CP-R133) were divided into a no-androgen group (Dihydrotestosterone (DHT): 0 nmol/L), very low-androgen group (DHT: 0.1 nmol/L), low-androgen group (DHT: 1 nmol/L), DHT = 10 nmol/L group, DHT (0 nmol/L) + ferrostatin-1 (Fer-1) group, DHT (0.1 nmol/L) + Fer-1 group, DHT (1 nmol/L) + Fer-1 group, DHT (10 nmol/L) + Fer-1 group. Cell viability, intracellular ferrous ion (Fe²⁺), malondialdehyde (MD

SUBMITTER: Shi HX 

PROVIDER: S-EPMC10401903 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature